Figure 5

Changes in mutation rate during the relapsing period with varying generation times. Mutation rate (mutations per (bp × generation)) is shown for generation times ranging from 18 to 320 h for each pair grouped by the number of years between the first and relapsed isolates. The dark blue line is obtained from the regressions and shows the estimated mutation rate for a given generation time (x-axis) and light blue regions show 95% confidence intervals. The first panel shows the relationship between mutation rate and generation time during early relapsing (in ≤ 2 years) based on n = 6 pairs. The second panel shows the relation between mutation rate and generation time for relapse in 2–14 years based on n = 12 pairs. In both panels the grey dashed vertical line is fixed at 18 h, and the horizontal line indicates the mutation rate of 2.798 × 10^–10 mutations per (bp × generation) as seen in early relapsing with generation times held constant at 18 h, fixed in both graphs for comparison availability.