Figure 3

Analysis of protein levels in PPO-KO cells. (a) Volcano plot of significantly differentially abundant proteins in PPO-KO cells compared to control cell lines. Cell lysates of two control (U-2 OS and N2) and two PPO-KO (R38/1 and W227/1) clones were digested with Lys-C/trypsin and resulting peptides identified and quantified using mass spectrometry-label free quantification analysis. In the volcano plot, the − log₁₀ (Benjamini–Hochberg corrected P value) is plotted against the log₂ (fold change: PPO-KO/control). Proteins significantly dysregulated in PPO-KO clones (localized above the curves drawn in Perseus with parameters FDR 0.05, s0 0.1.) are marked in red. (b) Expression levels of PPO (position marked by arrow) and ALAS-1 were determined by Western blotting of cell lysates separated by SDS-PAGE. Alpha-tubulin was used as a loading control.