Figure 4 | Scientific Reports

Figure 4

From: Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene

Figure 4

Analysis of OXPHOS (super)complexes. Protein complexes were extracted from cells by digitonin treatment and 20 µg of total proteins from each sample were separated by BN-PAGE and electroblotted onto a PVDF membrane. Antibodies specific for NDUFB8, SDHA, UQCRC2, MT-CO1 and ATP5F1B were used for detection of the complex CI, CII, CIII, CIV and CV, respectively. Signals of CII and CV were considered as loading controls.

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