Figure 3 | Scientific Reports

Figure 3

From: Identification of transcription factors dictating blood cell development using a bidirectional transcription network-based computational framework

Figure 3

Temporal TFs during normal erythroid differentiation. (a) A schematic overview of k-means clustering of the inferred influence scores for three timepoints: early (3.5 h), late (24 h), and definitive (96 h). The top arrows represent the three ANANSE-CAGE analyses necessary for the unsupervised clustering. The scatterplots are a schematic representation of the scatterplot generated by ANANSE-CAGE depicting influence score, an inferred score for how well differences in two cell states can be explained by a TF, and log2 fold change. (b) Scatterplot representing inferred influence scores and log2 fold change. The color and size of the individual dots represent an approximation for the number of target genes that are calculated from the number of edges in differential GRNs. The dotted line represents a visual cut-off for highlighting the top ranked TFs. (c) Heatmap showing seven clusters of TFs during erythroid differentiation. The value in each square represents the average influence score of each cluster at each timepoint. To the right nine TFs from each cluster is depicted. More TFs are summarized in Supplementary Table S2. The color intensity represents the average influence scores.

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