Figure 4 | Scientific Reports

Figure 4

From: Identification of transcription factors dictating blood cell development using a bidirectional transcription network-based computational framework

Figure 4

Key TFs in GSK-LSD1 induced early myeloid differentiation and immortalisation. (a) Bar graph depicting normalized (RPKM) CD86 expression (RNA-seq) in THP-1 after GSK-LSD1 induction (0 h, 8 h, and 24 h). (b) Differential expression of GSK-LSD1 induced THP-1 between 0 and 8 h (CAGE-seq). Y-axis depicts -log of the padj values and x-axis the log2 fold change. Dotted lines signify a cut-off for significance (padj < 0.05) and an absolute log2 fold change > 2. Genes significantly differentially expressed with a log2 fold change higher than 2 are depicted in red. (c) Scatterplot representing log2 fold change and inferred influence scores, an inferred score for how well differences in two cell states can be explained by a TF, in early myeloid differentiation. The color and size of the individual dots represent an approximation for the number of target genes that are calculated from the number of edges in differential GRNs. The dotted line represents a visual cut-off for highlighting the top ranked TFs. (d) Identification of TFs in THP-1 immortalisation, visualized similarly to (f).

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