Figure 5
From: Myeloid cell-specific ablation of Runx2 gene exacerbates post-infarct cardiac remodeling
Runx2 ablation impaired angiogenic activity of infiltrated myeloid cells following MI. (A–C) CD11b+ cells were prepared from Runx2fl/fl and Runx2 CKO hearts 7 days after MI and cultured for 24 h. Conditioned media from CD11b + myeloid cells of Runx2fl/fl and Runx2 CKO were added to HUVECs. After 8 h, tube formation of HUVECs was evaluated. (A) Representative images of tube formation are shown. The number of nodes (B) and branches (C) was measured. Data are shown as mean ± SD (n = 4 for each condition) *P < 0.05 vs Runx2fl/fl by student’s t-test. (D, E) CD11b+ F4/80+ macrophages were isolated from hearts of Runx2fl/fl and Runx2 CKO mice at 7 days after MI. Total mRNA was extracted from the cells and RNA-sequence was performed (n = 2 in each group). Pro-angiogenic factors were indicated by blue letters, while anti-angiogenic factors by red (D) Heat map shows the gene with > 2.0-fold change between Runx2fl//fl and Runx2 CKO with a P-value of < 0.05. (E) Volcano plots represent angiogenic gene expression compared Runx2fl/fl vs Runx2 CKO.
