Figure 6

Combination of CD4⁺, CD8⁺ conserved T cell epitopes and linkers in the multiepitope multivariant vaccine against SARS-CoV-2. 819 amino-acid residues organized in 39 CD8⁺ epitopes, joined by AAY linkers (dark grey), at the N-terminal region, followed by 16 CD4⁺ epitopes joined by GPGPG linkers (light grey), at the C-terminal, ended by a 6-HIS tail. The AAY and GPGPG spacers are included to avoid the formation of junctional epitopes³⁵. The epitopes belong to the ORF6 (pink), E (blue), S (yellow), ORF8 (red), M (magenta), N (green) and ORF1ab (cyan) SARS-CoV-2 proteins.