Figure 8
In silico cloning of the Multiepitope vaccine gene in a restriction cloning vector pET28b (+) in E.coli host. The cDNA nucleotide sequence of the vaccine was obtained with optimized codons for Escherichia coli K12 using the Java codon adaptation index tool. The GC content was 53.41%, while the normal range is 40–60%. Furthermore, the codon optimization index (CAI) value was 1.0. CAI higher than 0.5 is considered acceptable and indicates the high expression of the vector pET28b +. Here, the red areas indicate the Multiepitope vaccine DNA sequence, and the black areas represent the expression vector, pET28b (+).
