Figure 2

Time course profile of keratinase production (A) time course of decomposition of chicken feathers by B. velezensis NCIM 5802 (%), keratinase activity (U/mL), bacterial growth (OD600), soluble protein content (μg/mL), amino acid content (μg/mL) and pH (optimized culture conditions: initial pH 7.0, time 96 h, 3.5% v/v inoculum, 0.625% w/v substrate concentration at 40 °C and 200 rpm). Temperature and pH profile of keratinase produced by B. velezensis NCIM 5802 (B) pH optima and stability (C) temperature optima and stability. Values represent average of three independent replicates ± standard deviation.