Figure 2

Database analysis of miR-142-3p and RhoBTB3. Venn diagram depicts the mRNA targets of miR-142-3p predicted by three independent algorithms, Target Scan, DIANA, and miRDB (A). The expression of RhoBTB3 was reduced significantly in KIRC (B, P < 0.001). A negative correlation was established between miR-142-3p and RhoBTB3 in KIRC (C, P < 0.05). TargetScan software indicated that RhoBTB3 is a target gene of miR-142-3p (D). A luciferase reporter assay was conducted on 786-O cells to confirm the relative luciferase activity of RhoBTB3 after treatment with miR-142-3p mimics (E).