Figure 4 | Scientific Reports

Figure 4

From: MicroRNA-142-3p promotes renal cell carcinoma progression by targeting RhoBTB3 to regulate HIF-1 signaling and GGT/GSH pathways

Figure 4

miR-142-3p regulates RCC migration and invasion via hypoxia and GSH pathways by targeting RhoBTB3. 786-O cells were transfected with miR-control, miR-142-3p-mimics, and, miR-142-3p-inhibitors. Protein expression of RhoBTB3, hypoxia, and GSH pathway-related biomarkers, including HIF1A, VEGFA, and GGT1 using Western blot (A) (the original blots/gels are presented in Supplementary Fig. 3, the samples derive from the same experiment and that gels/blots were processed in parallel). The mRNA and protein expression of RhoBTB3, HIF1A, VEGFA, and GGT1 in 786-O cells was determined using qRT-PCR and Western blot, respectively (B,C). Expression of GGT1 differed significantly between RCC and the normal adjacent tissues (enhanced in KIRC, D, P < 0.001).

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