Figure 3
From: High-throughput 3D microvessel-on-a-chip model to study defective angiogenesis in systemic sclerosis
Response of the sprouted microvessels to cytokines and inhibitors. Sprouted microvessels were preincubated on day 8 with ALK5i or TNFα iAB for 90 min and subsequently exposed to (a combination of) TNFα or TGFß for 48 h in 2% (panel A and B) or 5% (panel C and D) human serum. (A) and (C) show representative images of cultures exposed to (a combination of) inhibitors and cytokines. (B) and (D) show quantification plots of the area covered by sprouts of exposed microvessels after exposure. The ratio of the sprouting area before and after the stability assay was normalized to the no exposure condition. n = 7–12, shown is the mean ± SEM, statistical test – ordinary one-way ANOVA (ns = not significant, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
