Figure 4
From: High-throughput 3D microvessel-on-a-chip model to study defective angiogenesis in systemic sclerosis
Exposure of sprouted HMVEC microvessels to sera from patients with systemic scleroderma. (A) Angiogenic sprouting was induced from day 4 till day 8 including 2% healthy human serum, followed by exposure for 48 h with 2% patient serum of 8 systemic scleroderma patients. Representative images before and after exposure are shown. (B) Bar graph presenting the ratio of the area covered by sprouts after versus before patient serum exposure. n = 3–6 shown is mean ± SEM. (C-E) Angiogenic sprouting was induced in the presence of 2% or 5% patient serum for 4 days. Subsequently, sprout stability was verified following four days of culture in absence of the sprouting cocktail with the addition of patient serum. Representative images are shown in (C), quantification of sprouting area at day 8 in (D) and the ratio of sprout stability in (E). n = 3–4, shown are mean ± SD.
