Table 4 List of plasmids and strains used in the study.

Escherichia coli DH5α

F-Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17

Invitrogen

Agrobacterium tumefaciens LBA4404

Ach5 with pAch5, Δtra Δocc ΔT_L ΔT_R Rif^r

³³

Pichia pastoris X-33

Wild type

Invitrogen¹⁸

Penicillium funiculosum NCIM1228

Cellulase-producing fungus obtained from NCIM (National Culture Collection Centre, Pune, India)

¹⁸

PfMig1⁸⁸

Catabolite derepressed strain of Penicillium funiculosum NCIM1228

³³

PfOAO6

Xylanase (Xyn5) overexpression in PfMig1⁸⁸ with the help of pOAO7 vector

This study

PfOAO7

Endo-β-1,3(4) glucanase (Egl16) overexpression in PfMig1⁸⁸ with the help of pOAO9 vector

This study

PfOAO8

Xylanase and endo-β-1,3(4) glucanase co-overexpression in PfMig1⁸⁸ with the help of pOAO10 vector

This study

Plasmids

pPICZαA

A zeocin-resistant vector for protein expression in P. pastoris under the control of AOX1 promoter

Genscript

pOAO6

A pPICZ_αA vector containing the PfGH5 gene under the control of AOX1 promoter

This study

pBIF

Kanamycin and hygromycin resistant vector containing EGFP

³⁷

pJET

Standard cloning vector containing the ampicillin-resistant gene

XB-VEC-1221267

pOAO7

pBIF vector containing the PfXyn5 gene along with its native promoter and terminator

This study

pOAO8

pJET vector containing the endo-β-1,3(4) glucanase gene along with its native promoter and terminator

This study

pOAO9

pBIF vector containing endo-β-1,3(4) glucanase gene along with its native promoter and terminator

This study

pOAO10

pBIF vector containing xylanase and endo-β-1,3(4) glucanase genes along with their native promoter and terminator

This study