Table 5 List of primers used in the study.

Screening of PfGH5 transformants in Pichia pastoris

AOX1-F (5′-GACTGGTTCCAATTGACAAGC-3′)

AOX1-R (5′-GCAAATGGCATTCTGACATCC-3′)

PfXyn5 and PfEgl16 plasmid construction cassette

PfXyn5-F (5′-TCCAGACTGCAGAACAGAGATCTCACGGTC-3′)

PfXyn5-R (5′-TTCCAGGATCCTTCTCTTCCGGTCCCAG-3′)

PfEgl16-F1 (5′-TCAGTGAGCTCAGTTGGCAGAAGACGAAGCAG-3′)

PfEgl16-R1 (5′-TATACACGCGTGGCGACGAATTCCGCGAC-3′)

PfEgl16-F2 (5′-TCAGTGGGCCCAGTTGGCAGAAGACGAAGCAG-3′)

PfEgl16-R2 (5′-TACTGGATCCGGCGACGAATTCCGCGAC-3′)

PgpdA-F (5′-GAATTCTGTACAGTGACCGGTGACTC-3′)

TrpC-R (5′-GCCAAGCTTCCTCTAAACAAGTGTACCTGTGC-3′)

PgpdA IR-F (5′-TGCGTCAGTCCAACATTTGT-3′)

RT-PCR primers

PfXyn5 RT F (5′-CTGGATCAGATTCCGTCGAAATTTTATTCGG-3′)

PfXyn5 RT R (5′-CCATCGGCTCCCCACAGATC-3′)

PfEgl16 RT F (5′-CTACAAGCAGCCCTAGTACTGTGACAG-3′)

PfEg16 RT R (5′-GTAAAGACGGGAGCAATGCTGCT-3′)

Tubulin F (5′-ATTGCTCAGGTTGTCTCCTCCATC-3′)

Tubulin R (5′-CATGGTGATCTCGTTGACAGAGTTGG-3′)