Figure 2
From: A molecular signature of lung-resident CD8+ T cells elicited by subunit vaccination
Comparative histologic and immunohistologic localisation of leukocytes responding to subunit vaccination and virus infection. (A) Mice were prime boost immunized i.p. or i.n. with L4R-b8r and αGC and lungs were harvested on d14 post boost. H&E staining of lungs from i.n. immunized mice showed prominent peri-vascular and peri-bronchiolar inflammatory infiltrate (lymphocytes and macrophages) compared to lungs of IP immunized mice. Data represent one of 3 lung sections per mouse from 3 mice per group. (B) Anti-CD3 staining of lung sections performed on d10—14 after booster immunization by i.p. or i.n. route as in Fig. 1. Arrows designate peri-vascular (PV), peri-bronchiolar (PB) and intravascular (IV) T cells. Scale bars, 50 μm. Data are representative of four sections per lung of two mice from two independent experiments per condition.
