Figure 3
Impact of L. acidophilus BIO5768 supplementation in a mouse model of C. rodentium infection. BIO5768 (5 × 108 CFU) was daily administered to C57BL/6J mice 5 days prior to infection and after the infection until the day of sacrifice. Mice were orally inoculated with 1 × 109 CFU C. rodentium DBS 120 K strain and sacrificed at either 4 or 9 days after infection. (A) Effect of BIO5768 on bacterial load of C. rodentium determined by plating over the course of the experiment, fecal samples dilution on Luria Bertani medium containing 50 µg/ml kanamycin and compared with control infected mice. (B) Effect of BIO5768 supplementation on the colon length. (C) Effect of BIO5768 supplementation on the crypt length. (D) qRT-PCR analysis of the expression of pro-inflammatory genes in the proximal colon of mice after 4 days post infection. (E) qRT-PCR analysis of the expression of pro-inflammatory genes in the distal colon of mice after 10 days post infection. Results are expressed as relative gene expressions (2−∆∆ct) values, by comparing the PCR cycle thresholds (Ct) for the gene of interest and for the house keeping gene β-actin (bact), compared with control mice. Results of a representative experiment out of 2 are expressed as means ± SEM of uninfected control mice (n = 10) and infected mice that are supplemented (n = 5) or not (n = 10) with BI05768. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.
