Figure 6
The expression profiling of selected gene candidates for Conium polyketide synthase 5 (CPKS5), polyketide reductase (PKR), l-alanine:5-keto-octanal aminotransferase (AAT), γ-coniceine reductase (CR), and S-adenosyl-l-methionine:coniine methyltransferase (CSAM) in planta. (a) The relative expression of CPKS5, PKR1, AAT1, CR1, and CSAM1 with housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GADPH) and cyclophilin 2 (CYP2). The relative expression is normalized using ACT and calculated using the 2−ΔΔCt-method and all values are compared to “leaf” within a plant (two biological replicates with three technical replicates, error bars are calculated using Eq. 1). (b) The number of the transcriptome reads for CPKS5, PKR1, AAT1, CR1, and CSAM1 with housekeeping genes ACT, GADPH and CYP2. The R package ggplot (https://ggplot2.tidyverse.org) was used to draw this figure.
