Table 1 Top in silico candidates for PKR, AAT, CR, and CSAM of coniine biosynthesis.
Enzyme | Transcript ID | E.C | PFAM | Size (bp) | Size (aa) | Size (kDa) | Nearest NCBI-BLAST hit |
|---|---|---|---|---|---|---|---|
PKR | PKR1: TRINITY_DN97220_c2_g4_i5 | 1.1.1.100; 2.3.1.85 | PF00106.23 short chain dehydrogenase; PF13561.4 Enoyl-(Acyl carrier protein) reductase; PF08659.8 KR domain | 1194 | 398 | 42.29 | Daucus carota subsp. sativus 3-oxoacyl-[acyl-carrier-protein] reductase 4-like (LOC108197291), mRNA |
PKR2: TRINITY_DN99958_c0_g2_i3 | 1.1.1.100; 2.3.1.85 | PF00106.23 short chain dehydrogenase; PF13561.4 Enoyl-(Acyl carrier protein) reductase; PF08659.8 KR domain | 963 | 321 | 33.39 | D. carota subsp. sativus 3-oxoacyl-[acyl-carrier-protein] reductase 4-like (LOC108197291), mRNA | |
AAT | AAT1: TRINITY_DN100028_c0_g1_i9 | 2.6.1.4; 2.6.1.2; 2.6.1.44; 2.6.1.12 | PF00155.19 Aminotransferase class I and II | 1443 | 481 | 53.27 | D. carota subsp. sativus glutamate–glyoxylate aminotransferase 2 (LOC108194271), transcript variant X1, mRNA |
AAT2: TRINITY_DN101579_c0_g1_i4 | 2.6.1.2; 2.6.1.12 | PF00155.19 Aminotransferase class I and II | 1638 | 546 | 60.01 | D. carota subsp. sativus alanine aminotransferase 2-like (LOC108222962), mRNA | |
AAT3: TRINITY_DN92259_c1_g1_i5/ TRINITY_DN92259_c1_g2_i1 | PF00202.19 Aminotransferase class-III | 1422 | 474 | 51.76 | D. carota subsp. sativus alanine–glyoxylate aminotransferase 2 homolog 1, mitochondrial (LOC108217775), transcript variant X1, mRNA | ||
AAT4: TRINITY_DN98702_c1_g1_i2 | 2.6.1.96; 2.6.1.62 | PF00202.19 Aminotransferase class-III | 1413 | 471 | 52.13 | D. carota subsp. sativus gamma aminobutyrate transaminase 2-like (LOC108200542), mRNA | |
AAT5: TRINITY_DN99413_c0_g2_i6 | 2.6.1.4; 2.6.1.2; 2.6.1.44; 2.6.1.12 | PF00155.19 Aminotransferase class I and II | 1437 | 479 | 52.81 | D. carota subsp. sativus glutamate–glyoxylate aminotransferase 2 (LOC108210226), mRNA | |
AAT6: TRINITY_DN99413_c0_g2_i8 | 2.6.1.4; 2.6.1.2; 2.6.1.44; 2.6.1.12 | PF00155.19 Aminotransferase class I and II | 1437 | 479 | 52.95 | D. carota subsp. sativus glutamate–glyoxylate aminotransferase 2-like (LOC108202675), transcript variant X1, mRNA | |
CR | CR1: TRINITY_DN86784_c0_g1_i2/ TRINITY_DN86784_c0_g2_i1 | PF13561.4 Enoyl-(Acyl carrier protein) reductase; PF00106.23 short chain dehydrogenase | 912 | 304 | 32.06 | D. carota subsp. sativus peroxisomal 2,4-dienoyl-CoA reductase-like (LOC108206087), mRNA | |
CR2: TRINITY_DN92324_c0_g1_i1 | PF13561.4 Enoyl-(Acyl carrier protein) reductase; PF00106.23 short chain dehydrogenase | 891 | 297 | 31.44 | D. carota subsp. sativus peroxisomal 2,4-dienoyl-CoA reductase (LOC108224824), mRNA | ||
CR3: TRINITY_DN92324_c0_g1_i3 | PF13561.4 Enoyl-(Acyl carrier protein) reductase; PF00106.23 short chain dehydrogenase | 741 | 247 | 26.03 | D. carota subsp. sativus peroxisomal 2,4-dienoyl-CoA reductase (LOC108224824), mRNA | ||
CR4: TRINITY_DN93222_c2_g6_i1 | 1.3.1.33; 1.1.1.2 | PF00106.23 short chain dehydrogenase | 732 | 244 | 27.19 | D. carota subsp. sativus short-chain dehydrogenase TIC 32, chloroplastic-like (LOC108211237) | |
CR5: TRINITY_DN93586_c1_g1_i1 | 1.1.1.330; 1.3.1.33 | PF00106.23 short chain dehydrogenase | 945 | 315 | 34.29 | D. carota subsp. sativus short-chain dehydrogenase TIC 32, chloroplastic-like (LOC108196149), mRNA | |
CR6: TRINITY_DN93845_c0_g1_i3 | 1.3.1.33; 1.1.1.330 | PF00106.23 short chain dehydrogenase | 636 | 212 | 22.86 | D. carota subsp. sativus short-chain dehydrogenase TIC 32, chloroplastic-like (LOC108199832), mRNA | |
CR7: TRINITY_DN95493_c4_g1_i3 | 1.1.1.330; 1.3.1.33 | PF00106.23 short chain dehydrogenase | 771 | 257 | 28.25 | D. carota subsp. sativus short-chain dehydrogenase TIC 32, chloroplastic-like (LOC108211237) | |
CSAM | CSAM1: TRINITY_DN100232_c1_g3_i3 | PF03492.13 SAM dependent carboxyl methyltransferase | 399 | 133 | 15.21 | D. carota subsp. sativus benzoate carboxyl methyltransferase-like (LOC108212050), mRNA | |
CSAM2: TRINITY_DN109295_c0_g1_i1 | 492 | 164 | 18.78 | No hits | |||
CSAM3: TRINITY_DN83407_c0_g2_i2 | PF03492.13 SAM dependent carboxyl methyltransferase | 474 | 158 | 17.82 | D. carota subsp. sativus benzoate carboxyl methyltransferase-like (LOC108202053), mRNA | ||
CSAM4: TRINITY_DN91104_c0_g1_i3 | PF03492.13 SAM dependent carboxyl methyltransferase | 1107 | 369 | 41.43 | D. carota subsp. sativus salicylate carboxymethyltransferase-like (LOC108225466), mRNA | ||
CSAM5: TRINITY_DN91104_c0_g1_i9 | PF03492.13 SAM dependent carboxyl methyltransferase | 669a | 223a | 25.37a | D. carota subsp. sativus salicylate carboxymethyltransferase-like (LOC108225576), mRNA | ||
CSAM6: TRINITY_DN95275_c3_g1_i1/ TRINITY_DN99752_c3_g3_i3 | PF03492.13 SAM dependent carboxyl methyltransferase | 1071 | 357 | 40.43 | D. carota subsp. sativus salicylate carboxymethyltransferase-like (LOC108194344), mRNA | ||
CSAM7: TRINITY_DN95333_c0_g1_i17 | PF03492.13 SAM dependent carboxyl methyltransferase | 438 | 146 | 16.39 | D. carota subsp. sativus benzoate carboxyl methyltransferase-like (LOC108212050), mRNA |
