Table 1 Comparison of distribution statistics and expression variation of transcripts proposed as HK in R. arenarum stage 25 larvae.

From: Hypothesis-driven dragging of transcriptomic data to analyze proven targeted pathways in Rhinella arenarum larvae exposed to organophosphorus pesticides

Gene ID

Gene name

Mean

Minimum

Maximum

SD

Median

CV %

ef1a0

Elongation factor 1-alpha, somatic form

7329.9

6610.7

8457.7

586.4

7265.4

8.00

ef1b

Elongation factor 1-beta

583.4

498.6

676.9

64.3

589.4

11.03

ef1d

Elongation factor 1-delta

922.1

800.7

1074.7

98.3

910.4

10.66

ef1g-a

Elongation factor 1-gamma-A

2993.7

2611.2

3344.1

252.9

2968.2

8.45

g3pdh

Glyceraldehyde-3-phosphate dehydrogenase

1822.0

1289.5

2425.1

411.3

1768.3

22.58

rl8

60S ribosomal protein L8

1108.0

883.6

1309.3

134.8

1131.6

12.16

tuba

Tubulin alpha chain

1829.9

1589.5

2014.7

142.9

1826.3

7.81

tuba1

Tubulin alpha-1 chain

146.1

98.1

195.5

31.8

142.1

21.74

tubb

Tubulin beta chain

518.4

350.2

586.8

70.4

526.8

13.58

tubb4b

Tubulin beta-4B chain

219.0

137.1

253.7

33.3

224.5

15.19

actb

Actin, cytoplasmic 1

363.6

301.7

394.4

37.2

380.3

10.22

acta4

Actin, alpha sarcomeric/skeletal

90.1

15.1

259.4

73.3

61.9

81.27

  1. Data calculated from massive RNA-seq and transcriptomic analysis are expressed as TMM values. A limit of 20% for the CV is proposed to consider a transcript as an HK gene in expression analysis.