Figure 5
From: Cannabidiol improves Nile tilapia cichlid fish welfare
Ex vivo effects of 0 (control), 1 (CBD 1), 10 (CBD 10) and 20 mg/kg (CBD 20) of cannabidiol (CBD) on (a) gonadosomatic index (GI) of Nile tilapias (n = 10) and (b) the mean spermatozoa (SPZ) number by field (total of 20 fields by fish) in testicular explant from Nile tilapias (n = 5). Fish were exposed for 35 days to the CBD. Different uppercase letters indicate significant differences between treatments (Tukey HSD test, p < 0.05). The number of SPZs was determined by morpho-histological analysis using ImageJ software. Values are mean ± SEM. (c,d,e,f) Histological sections of Nile tilapia testis following 35-days of exposure to CBD. Spermatozoa areas are indicated by SPZ in each figure (scale: 50 μm).
