Figure 1

Transcriptomics of pro-growth RGC states predict Elk-1 and REST as top positive and negative transcription factor regulators. (a) Retinas were treated with either a regenerative cocktail of CNTF, CPT-cAMP, oncomodulin, and an shRNA targeting PTEN, or a control injection. After either 1 or 5 days after optic nerve injury, tissue was collected for subsequent RGC RNA-sequencing. In parallel, RGCs were purified by immunopanning from either E18 or P5 mice and subsequently collected for RNA sequencing. Cells were FACS-sorted for Thy1 + and sequenced. (b) The transcriptome of all 3 sample types were evaluated with GSEA to identify likely upstream transcription factors, and averaged. The top 10 positive and negative transcription factors by average EdgeR score are presented. (c) Enrichment plots with normalized enrichment score (NES) are presented for the top positive and negative predicted transcription factors.