Figure 7 | Scientific Reports

Figure 7

From: Endothelin receptor B enhances liver injury and pro-inflammatory responses by increasing G-protein-coupled receptor kinase-2 expression in primary biliary cholangitis

Figure 7

EDNRB knockdown or inhibition alleviated liver pathologic damage by suppressing GRK2 expression and inactivating NF-κB pathway in PBC mice. (A) HE staining of liver tissues of mice injected with normal saline (NaCl), control adenoviruses (Control), Ad-shEDNRB adenoviruses (Si-EDNRB), or EDNRB inhibitor Bosentan (EDNRB inhibitor). Each group contained 10 mice. (B–F) The mRNA levels of EDNRB, GRK2, NF-κB, IKKα, and IKKβ were measured with RT-qPCR assay in liver samples of mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses, or EDNRB inhibitor Bosentan. (G) The protein levels of EDNRB, GRK2, NF-κB, IKKα, IKKβ, p-NF-κB, p-IKKα and p-IKKβ were detected via western blotting technique in liver tissues of mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses, or EDNRB inhibitor Bosentan. (H) Levels of EDNRB, GRK2, NF-κB, IKKα, and IKKβ proteins were measured through IHC assay in liver tissues of mice injected with normal saline, control adenoviruses, Ad-shEDNRB adenoviruses, or EDNRB inhibitor Bosentan.

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