Figure 2 | Scientific Reports

Figure 2

From: Naturally-aged microglia exhibit phagocytic dysfunction accompanied by gene expression changes reflective of underlying neurologic disease

Figure 2

Aged microglia have less uptake capacity of Aβ overtime than their younger counterparts. Microglia from 3 young (2 months) or old (22 months) female mice were isolated, combined with their age-matched counterparts, and divided into 5 time points where they were incubated with 2.5 μM of Aβ42 for 1, 3, 6, and 12 h. Microglia were analyzed via flow cytometry to measure phagocytosis. (a) Representative flow plots of 1 replicate showing uptake of Aβ42 by microglia over time. (b) % of microglia that are Aβ42 positive over time. Dashed lines indicate line of best fit. (c) Geometric mean of Aβ42 in Aβ42+ microglia. Dashed lines indicate line of best fit. Simple linear regression was used to determine differences between the lines of best fit.

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