Figure 3 | Scientific Reports

Figure 3

From: Naturally-aged microglia exhibit phagocytic dysfunction accompanied by gene expression changes reflective of underlying neurologic disease

Figure 3

Pathway analysis of young and old microglia that either did or did not take up peptide showed deficiencies of old microglia. Young (2 months) and old (18 months) microglia (3 male mice were combined to make one replicate; this was repeated for a total of 4 replicates/group) were incubated with 2.5 μM of Aβ42 for 2 h and then were FACSorted based on Aβ42 uptake. Sorted microglia’s RNA was isolated and used to run bulk RNAseq analysis. (a) Schematic of phagocytosis assay and flow sorting, images made using ©BioRender - biorender.com. (b) Principal component analysis plot of the four different microglia groups (Young Aβ42+, Young Aβ42−, Old Aβ42+, Old Aβ42−) with 4 replicates each. Pathway analysis of DEGs in both the young and old (c) Aβ42+ and (d) Aβ42− microglia graphed based on their p value. The number of DEGs in each pathway are indicated in parentheses.

Back to article page