Figure 4

CLSM images of C. albicans in planktonic (a) and biofilm form (b) incubated with PRE (4 mg/mL), Zn (II) (1.8 mg/mL) and PRE and Zn (II) combined (4 mg/mL + 1.8 mg/mL) for 24 h. Blue channel shows calcofluor in C. albicans cells walls and green channel shows ROS production in C. albicans cells. Graphs shows ROS levels for C. albicans in planktonic form (c) and biofilm form (d) normalised to total biomass. Significance indicated by *, where **p < 0.01, ***p < 0.001, and ****p < 0.0001. Data represented as mean ± SEM (n = 3).