Figure 3 | Scientific Reports

Figure 3

From: Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting

Figure 3

SARS-CoV-2 RT-PCR multiplex assay development. Assay detects 2 sequences (N1 and N2) from SARS-CoV-2 and a human RNA (RP) as an internal extraction control. (A) Mock clinical samples created by spiking SARS-CoV-2 synthetic RNA at the level of 1 × LoD into individual negative nasopharyngeal samples. N = 20 per technical replicate. The black lines denote the means for each target and replicate. The red line denotes the Ct cutoff (38) for a positive result. (B) 5 negative and 5 positive clinical samples were assessed and compared to the singleplex detection. Samples were blinded to assay personnel. The red line denotes the Ct cutoff (38) for a positive result.

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