Figure 4 | Scientific Reports

Figure 4

From: Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting

Figure 4

5-sample pool SARS-CoV-2 RT-PCR assay development. (A) Analysis of the first 103 positive samples from 2021 binned to visualize N1 and N2 Ct level. (B) Pools of 5 samples containing one positive sample per pool. Positive samples were chosen to represent the occupational sample population (14 low Ct samples) and to examine the effect of 5 sample pools on high Ct samples (11 samples), which could be missed due to the Ct delay caused by pooling.

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