Figure 4

5-sample pool SARS-CoV-2 RT-PCR assay development. (A) Analysis of the first 103 positive samples from 2021 binned to visualize N1 and N2 Ct level. (B) Pools of 5 samples containing one positive sample per pool. Positive samples were chosen to represent the occupational sample population (14 low Ct samples) and to examine the effect of 5 sample pools on high Ct samples (11 samples), which could be missed due to the Ct delay caused by pooling.