Figure 6 | Scientific Reports

Figure 6

From: Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting

Figure 6

Proposed efficient hybrid testing design using two teams to perform both multiplex testing and pooled testing simultaneously. Day 1 denotes the first day of any testing run, for example a Monday or a week. To maintain ~ 24 time to results, all higher risk samples such as symptomatic, known prior contact with a positive individual and recent travel would be run using the multiplex testing. Regular screening would be run by a second team using the 5-sample pooling method. Negative samples from the screening population would be reported the same day. Samples from the positive pools would be set aside and run with the Day 2 multiplex samples. In this way, all samples would report out in ~ 48 h and all high-risk samples would report out in ~ 24 h from collection.

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