Table 2 Overview of analysis methods.

From: Singleplex, multiplex and pooled sample real-time RT-PCR assays for detection of SARS-CoV-2 in an occupational medicine setting

Method

Number of patient samples/96-well plate

Time per run, manual extraction

Time per run, automated extraction

3 target singleplex PCR

29

4–5 h*

3 h

3 target multiplex PCR

93

6–7 h*

3 h

5-sample pool combined with 3 target singleplex PCR

145

4–5 h*

3–4 h#

  1. *Manual extraction allows extraction of 23 samples at a time at ~ 1 h per round. # Automated extraction for samples combined prior to RNA extraction which allows for extraction of 96 samples at a time.
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