Figure 3
Spatial correlation within lineages grown under glucose-deficient conditions. Three-dimensional cellular lineages of E. coli acquired under (A) control conditions (22.2 mM glucose) and (B) glucose-deficient conditions (0.1 mM glucose) obtained by triplicate experiment. Individual E. coli cells were color-coded: red, high intracellular ATP concentration; blue, low intracellular ATP concentration. Individual P values for spatial localization are shown. (C) Effects that contribute to spatial localization28 of ATP classes. By quantifying the average difference in phenotype among cells closest to a specific individual cell (dNB) and the sister cell of that closest cell (dER), we assessed whether spatial proximity contributed to spatial correlation (dER/dNB). However, for lineage factors, we measured the average difference in phenotype between the closest relative of each individual cell (dCR) and a cell at an equidistant position (dED). The larger the phenotypic difference of the cell at an equidistant position, the stronger the effect of lineage in promoting spatial correlation. The error bar shows the 95% confidence interval in a t-distribution. *, P < 0.05; H0, dER/dNB = 1 or dED/dCR = 1.
