Table 1 The acute toxicity of sulfoxaflor and Amistar to B. terrestris and O. bicornis across castes and sexes.

From: Intra-specific variation in sensitivity of Bombus terrestris and Osmia bicornis to three pesticides

Sex/caste

Timepoint (h)

µg/bee (CI)

ng/mg (CI)

Sulfoxaflor LD50 Bombus terrestris ssp. terrestris contact

Worker

48

6.323 (4.672, 7.975)

26.741 (19.771, 33.71)

Male

72

0.6 (0.399, 0.801)

1.821 (1.214, 2.428)

Queen

96

37.513 (28.775, 46.25)

48.038 (36.867, 59.208)

Sulfoxaflor LD50 Bombus terrestris ssp. audax oral

Worker

48

0.126 (0.12, 0.133)

0.606 (0.581, 0.631)

Male

48

0.08 (0.066, 0.095)

0.35 (0.289, 0.411)

Queen

96

0.452 (0.369, 0.535)

0.731 (0.605, 0.857)

Amistar LD50 Bombus terrestris ssp. audax oral

Worker

96

252.3 (201.6, 303.0)

1043.2 (837.7, 1248.7)

Male

72

157.6 (128.2, 186.9)

549 (448.913, 649.0)

Queen

96

622.6 (473.7, 771.6)

836.9 (639, 1034.8)

Sulfoxaflor LD50 Osmia bicornis contact

Female

96

0.051 (0.032, 0.069)

0.567 (0.358, 0.775)

Male

96

0.029 (0.018, 0.041)

0.679 (0.413, 0.946)

Sulfoxaflor LD50 Osmia bicornis oral

Female

48

0.013 (0.007, 0.018)

0.140 (0.081, 0.199)

Male

48

0.01 (0.007, 0.013)

0.209 (0.147, 0.271)

Amistar LD50 Osmia bicornis oral

Female

48

578.8 (324.4, 833.2)

5324 (2902.9, 7565.2)

Male

48

407.4 (258.5, 556.4)

6715.8 (4314.4, 9117.3)

  1. LD50 values and related 95% confidence intervals were calculated at timepoints corresponding to the steady-state mortality except in the case of O. bicornis oral exposure to sulfoxaflor and Amistar (see methods section). All endpoints are expressed as doses, normalised or not by body weight.