Figure 9 | Scientific Reports

Figure 9

From: Continuous exposure to isoprenaline reduced myotube size by delaying myoblast differentiation and fusion through the NFAT-MEF2C signaling pathway

Figure 9

NFATs are involved in inhibiting C2C12 myoblast cell differentiation/fusion by ISO through coordination with MEF2C. Myoblast cells were stimulated by continuous single-dose ISO under DM for six days and then stained with MyoG or MEF2C 24 h after the cells were transfected with Ad-NFATc1, Ad-NFATc2, Ad-shNFATc3 or Ad-shNFATc4 at 100 MOI. Blue: DAPI-stained nuclei; Red: MEF2C. (B) The percentage of MyoG- or MEF2C-positive nuclei within the total nucleus was analyzed. n = 3, @P < 0.05 versus Ctrl; *P < 0.05 versus ISO group; &P < 0.05 versus ISO + Ad-NFATc1 group; $P > 0.05 versus ISO + Ad-NFATc1 group; #P < 0.05 versus ISO + Ad-NFATc1 group. (C) Quantitative assay for the number of MyoG or MEF2C + nuclei in the cells with fewer than 3 nuclei from 9A. n = 3, @P < 0.05 versus Ctrl; *P < 0.05 versus ISO group;&P < 0.05 versus ISO + Ad-NFATc1 group; #P < 0.05 versus ISO + Ad-NFATc1 group. (D) Quantitative assay for the number of MyoG or MEF2C + nuclei in the cells with more than 3 nuclei from 9A. n = 3, @P < 0.05 versus Ctrl; *P < 0.05 versus ISO group;&P < 0.05 versus ISO + Ad-NFATc1 group; #P < 0.05 versus ISO + Ad-NFATc1, ISO + Ad-NFATc2 or ISO + Ad-shNFATc3 group.

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