Figure 3 | Scientific Reports

Figure 3

From: Tumor suppressor DEAR1 regulates mammary epithelial cell fate and predicts early onset and metastasis in triple negative breast cancer

Figure 3

The effect of DEAR1-KD on luminal and myoepithelial/basal surface marker expression in HMEC cells. (a) Images at ×10 magnification of staining of 76N-E6 control (CshR) and DEAR1-KD (DshR) clones grown in 3D mammosphere culture in the absence (top) or presence (bottom) of TGF-β with luminal and myoepithelial/basal cytokeratin surface markers, CK 8/18 (red) and CK 5 (green), respectively. Fluorescence images were taken using OLYMPUS cellSens Software and scale bars equal 100 µm. (b) FACS data showing proportion of cells that express EpCAM and CD49f from 2 CshR clones (top) and 2 DshR clones (bottom). (c) Bar plots showing the mean ± SD percentage of cells displaying luminal (EpCAM+/CD49f+) and basal (EpCAM−/CD49f+) surface markers from CshR (orange) and DshR (teal) clones. p-values were calculated using a two-tailed Student t-test between groups. (d) Bar plots quantifying FACS data assaying CD24 and CD44 protein expression from CshR (orange) and DshR (teal) clones. p-values were calculated using a two-tailed Student t-test between groups.

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