Figure 2 | Scientific Reports

Figure 2

From: Nestin is a marker of unipotent embryonic and adult progenitors differentiating into an epithelial cell lineage of the hair follicles

Figure 2

EGFP+ cells with characteristics of epithelial cells in Nes-Cre/CAG-CAT-EGFP mouse embryos during the hair peg stage. (a) Time-course analysis of EGFP expression in epithelial cells of embryonic HFs in Nes-Cre/CAG-CAT-EGFP mice. Skin collected at germ (A), advanced germ (B), peg (C, D), and bulbous peg (E) stages was subjected to immunolabeling for EGFP (green) and laminin (white). Scale bars, 10 μm (A, B, C, E), 5 μm (D). (b) Immunolabeling for EGFP (A, E, I, M, R), K5 (B), VIM (F), SOX2 (J), SOX10 (N), and S100A6 (S) in hair peg epithelia. Nuclei were counterstained by Hoechst 33258 (C, G, K, O, T). A subset of cells in anagen hair bulbs, presumably melanocytes, were immunolabeled for SOX10 (Q). Scale bars, 10 μm (D, H, L, P, U), 20 μm (Q).

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