Figure 5
From: RELA is required for CD271 expression and stem-like characteristics in hypopharyngeal cancer
Deletion of the RELA-binding site reduces sphere-formation activity in HPCM2 cells. (A) Sequence chromatograms of cells harboring deletion of site + 1045 (DEL). Red characters indicate protospacer adjacent motif sequences, and green characters indicate the + 1045 RELA-binding site. (B) CD271 expression according to flow cytometry. The numbers in the histogram indicate the mean fluorescence intensity of phycoerythrin-conjugated anti-CD271. (C) Sphere-formation activity of RELA binding site-knock out HPCM2 cells according to MTT assay. DEL, cells harboring deletion of the RELA-binding site (+ 1045); EV, empty vector (control cells). Right panels show the representative images of the spheroid cells. (D) Cell proliferation assay. EV and DEL HPCM2 cells were seeded (1000 cells/well) and cultured and cell numbers were determined by MTT assay. (E) GSEA analysis. The exression microarray data of EV versus DEL were compared using GSEA software. A gene set of keratinization was downregulated in EV cells.
