Table 2 The CCD data with input variables and output responsesa,b.
Run | Factor 1 | Factor 2 | Factor 3 | Factor 4 | Response 1 | Response 2 | Response 3 | Response 4 | Response 5 |
|---|---|---|---|---|---|---|---|---|---|
A: pH of the Buffer | B: Initial Gradient (% MP-A) | C: Flow Rate (mL/min) | D: Column Temperature (°C) | R1 | R2 | R3 | R4 | R5 | |
1 | 4.3 | 65 | 0.6 | 30 | 5.8 | 5.1 | 2.2 | 1.2 | 3.9 |
2 | 4.0 | 80 | 0.4 | 40 | 8.1 | 4.0 | 4.2 | 1.3 | 7.1 |
3 | 4.0 | 65 | 0.5 | 40 | 7.5 | 3.6 | 3.4 | 0.0 | 5.8 |
4 | 4.3 | 80 | 0.5 | 40 | 1.6 | 7.0 | 0.0 | 2.6 | 0.0 |
5 | 4.0 | 80 | 0.5 | 40 | 6.5 | 3.4 | 0.0 | 4.1 | 6.7 |
6 | 3.7 | 65 | 0.4 | 30 | 13.8 | 0.0 | 3.6 | 6.5 | 6.6 |
7 | 4.0 | 95 | 0.5 | 40 | 6.9 | 3.7 | 0.0 | 3.6 | 6.1 |
8 | 4.0 | 80 | 0.5 | 30 | 9.2 | 1.4 | 3.8 | 2.6 | 6.1 |
9 | 3.7 | 65 | 0.4 | 50 | 10.5 | 1.7 | 4.4 | 3.0 | 7.7 |
10 | 4.0 | 80 | 0.5 | 50 | 2.8 | 6.0 | 0.0 | 4.2 | 3.6 |
11 | 3.7 | 95 | 0.4 | 30 | 13.5 | 0.0 | 3.7 | 7.7 | 6.7 |
12 | 4.3 | 65 | 0.4 | 30 | 7.3 | 5.4 | 0.0 | 2.7 | 4.7 |
13 | 4.3 | 95 | 0.6 | 30 | 4.9 | 5.0 | 1.7 | 1.4 | 3.8 |
14 | 3.7 | 95 | 0.6 | 30 | 10.8 | 0.0 | 3.5 | 4.2 | 6.5 |
15 | 4.3 | 95 | 0.4 | 50 | 0.0 | 10.1 | 0.0 | 0.0 | 2.3 |
16 | 4.3 | 95 | 0.6 | 50 | 0.0 | 5.9 | 0.0 | 0.0 | 2.8 |
17 | 4.3 | 95 | 0.4 | 30 | 8.2 | 5.6 | 2.3 | 0.0 | 4.9 |
18 | 4.3 | 65 | 0.4 | 50 | 1.5 | 9.7 | 0.0 | 0.0 | 0.0 |
19 | 3.7 | 95 | 0.4 | 50 | 10.6 | 1.9 | 4.5 | 2.9 | 7.8 |
20 | 3.7 | 95 | 0.6 | 50 | 7.1 | 1.2 | 0.0 | 3.9 | 7.3 |
21 | 4.0 | 80 | 0.6 | 40 | 5.8 | 3.0 | 3.2 | 1.1 | 6.2 |
22 | 4.3 | 65 | 0.6 | 50 | 0.0 | 0.0 | 0.0 | 0.0 | 3.0 |
23 | 3.7 | 65 | 0.6 | 50 | 8.6 | 1.4 | 0.0 | 0.0 | 7.5 |
24 | 4.0 | 80 | 0.5 | 40 | 10.1 | 3.3 | 0.0 | 4.1 | 6.8 |
25 | 3.7 | 65 | 0.6 | 30 | 14.1 | 0.0 | 3.4 | 4.2 | 6.5 |
26 | 3.7 | 80 | 0.5 | 40 | 15.3 | 0.0 | 4.0 | 3.2 | 7.3 |
27 | 4.0 | 80 | 0.5 | 40 | 9.9 | 3.3 | 0.0 | 4.1 | 6.7 |
