Figure 3 | Scientific Reports

Figure 3

From: Immune mobilising T cell receptors redirect polyclonal CD8+ T cells in chronic HIV infection to form immunological synapses

Figure 3

Comparison of ImmTAV-mediated immunological synapses formed by CD8+ T cells from HIV-naïve subjects and PLWH. SL9-pulsed T2 cells were cultured with HIV-naïve donor (HD) CD8+ T cells (n = 3) or SL9 peptide pre-stimulated PLWH CD8+ T cells (n = 3) at a ratio of 1:1 in the presence or absence of m121 ImmTAV (0.5 nM). (a) Quantification of conjugates formed after 15 min with pre-stimulated PLWH ± ImmTAV (n > 50 targets counted/slide, blinded analysis) and plotted as mean ± SD). (b) Quantification of MTOC distance from the IS (µM; data were analysed by one-way ANOVA with correction for multiple comparisons) and (c) % conjugates with distal, dispersed or docked expression of perforin in HD and PLWH CD8+ T cells. At least 10 conjugates per subject and per condition were analysed and data were pooled.

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