Figure 5
From: An increase in mitochondrial TOM activates apoptosis to drive retinal neurodegeneration
DmHsp22 is upregulated in a dose-dependent manner and associates with Tom40. (a) Silver-stained 15% SDS-PAGE showing affinity-purified (anti-FLAG) Tom40 samples from eye membranes solubilised with digitonin. Each sample corresponds to protein derived from ~ 800 fly heads and is representative of at least 3 independent samples. Affinity purified products from flies reared at 25 °C, carrying one (lane 1) or two (lane 2) copies of the UAS.Tom40 transgene. The boxed area highlights Hsp22. (b) Quantification of DmHsp22 mRNA in flies expressing 0, 1 or 2 copies of UAS.Tom40 at 25 °C.
