Figure 2

Optimized 3d cell culture protocol for implementing 3D-ASM. (A) Cell and matrigel mixture dispensed on the surface of the 384-pillar plate by 3D-Cell spotter. (B). The 384-pillar plate aggregates the cells into one place through storage in an ice box for 2 h while combined with the wet chamber. (C). In the CO₂ incubator condition, over gelation occurs if the wet chamber and the 384-pillar plate are not combined. (D). In humidified incubator and wet chamber conditions, the 3D-cell mixture is stabilized after gelation for 20 min. (de-gelation occurs at the 5-min condition, and over gelation occurs at the 30-min condition).