Figure 4
From: Optimization of 3D-aggregated spheroid model (3D-ASM) for selecting high efficacy drugs

Confocal image scan and 3D deconvolution image implementation for 3D-ASM. (A) Serial Z-stack confocal imaging and 3d deconvolution image implementation after cell nucleus staining (blue). (B). Serial Z-stack confocal imaging and 3d deconvolution image implementation after cytoskeletal staining (green). (C). Implementation of serial Z-stack confocal image and 3d deconvolution image by merging cell nucleus and cytoskeleton images (blue and green merged).