Table 1 Biophysical parameters of Ca_v1.2 WT, R412M and G406R channels.

Activation parameters

(n = 18)

(n = 10)

(n = 5)

 Peak density (pA/pF)

− 12.3 ± 0.95

− 10.9 ± 0.75

− 11.0 ± 1.7

 V_h (mV)

1.9 ± 1.3

− 3.7 ± 1.3*

− 9.2 ± 1.2^†

 k

7.8 ± 0.23

5.7 ± 0.23*

5.1 ± 0.40^†

Conductance parameters

(n = 18)

(n = 10)

(n = 5)

 V_rev (mV)

72.5 ± 1.3

62.8 ± 2.1*

60.8 ± 2.0^†

 Peak density (pS)

0.26 ± 0.025

0.22 ± 0.056

0.17 ± 0.013

Inactivation parameters

(n = 15)

(n = 12)

(n = 6)

 I_Ca V_h (mV)

− 11.8 ± 1.4

− 4.1 ± 1.4*

− 10.0 ± 2.0

 I_Ca k

− 8.2 ± 1.1

− 7.8 ± 0.65

− 6.6 ± 0.52^†

 I_Ba Vh (mV)

− 21.7 ± 6.3

− 12.1 ± 5.9 *

NA

 I_Ba k

− 12.9 ± 2.9

− 12.1 ± 4.7

NA

Inactivation

(n = 18)

(n = 10)

(n = 5)

 τ_fast (ms)

0 mV

18.7 ± 2.0

24.0 ± 1.8

35.8 ± 4.8^†

+ 10 mV

15.0 ± 0.8

25.5 ± 1.6*

38.0 ± 7.2^†

+ 20 mV

15.6 ± 0.7

31.0 ± 2.7*

39.9 ± 2.3^†

 A fast/A peak

0 mV

0.51 ± 0.015

0.55 ± 0.033^‡

0.41 ± 0.034

+ 10 mV

0.52 ± 0.0092

0.50 ± 0.012^‡

0.29 ± 0.044^†

+ 20 mV

0.46 ± 0.010

0.40 ± 0.013

0.18 ± 0.019^†

 τ_slow (ms)

0 mV

165.5 ± 26.6

400.0 ± 102.8

387.7 ± 161.3

+ 10 mV

123.4 ± 6.6

347.5 ± 75.3*

346.6 ± 135.4

+ 20 mV

105.0 ± 5.6

251.3 ± 39.5*

244.6 ± 37.8^†

 A slow/A peak

0 mV

0.37 ± 0.010

0.23 ± 0.017*

0.22 ± 0.031^†

+ 10 mV

0.40 ± 0.0063

0.31 ± 0.022*

0.30 ± 0.025^†

+ 20 mV

0.48 ± 0.010

0.42 ± 0.021

0.34 ± 0.019^†

 A steady/A peak

0 mV

0.15 ± 0.020

0.30 ± 0.039*

0.42 ± 0.013^†

+ 10 mV

0.082 ± 0.0081

0.21 ± 0.034*

0.45 ± 0.048^†

+ 20 mV

0.055 ± 0.0057

0.60 ± 0.39*

0.48 ± 0.023^†