Figure 2

Analysis of FXR mRNA, Bsep mRNA, Ntcp mRNA and NF-κBp65 mRNA expression by RT-PCR. Through RT–PCR, we found that in the treatment group, model group, control group and sham operation group, there were significant difference among the four groups in the ratios of FXR/GAPDH, FXR/GAPDH, FXR/GAPDH and NF-κBp65/GAPDH. In the model group, FXR and Bsep decreased significantly compared with the control group, while Ntcp and NF-κBp65 increased significantly. After GW4064 treatment, FXR and Bsep in the treatment group increased compared with the model group, while Ntcp and NF-κBp65 continued to decrease, inflammation was controlled, cholestasis was improved, and the situation was developing in a better direction.