Figure 1
From: Optogenetic restoration of high sensitivity vision with bReaChES, a red-shifted channelrhodopsin
Prolonged expression of bReaChES in the retinal ganglion cells of wild-type (WT) and dystrophic (Dys) mouse retinae. (A–D) Representative fundus images (left: color, right: short-wavelength fluorescent fundus photography) of WT and dystrophic mice 6 (A,C) and 28 (B,D) weeks post-treatment. Green fluorescence indicates bReaChES-eYFP expression. (E–H) Representative WT retinal section (E) and flatmount (F–G) images that show membrane expression of bReaChES in GCL, IPL and nerve fibers. Representative retinal flatmounts from WT (H) and dystrophic (I) mouse retinae that show membrane expression of bReaChES in individual retinal ganglion cells. (J) The transduction rate of optogenetic vector in RGL is not significantly different between WT and Dys mice 6 months post injection (N = 5 WT and N = 4 Dys). (K) The density of RGL measured is not significantly different between WT and Dys mice 6 months post injection (N = 5 WT and N = 4 Dys). Error bars indicate ± SD. White scale bar = 50um. (Abbreviations: eYFP = enhanced yellow fluorescence protein; PI = propidium iodide; GCL = ganglion cell layer; IPL = inner plexiform layer; INL = inner nuclear layer; OPL = outer plexiform layer; ONL = outer nuclear layer).
