Figure 2
From: Optogenetic restoration of high sensitivity vision with bReaChES, a red-shifted channelrhodopsin
bReaChES expression in dystrophic mouse retina confers novel light sensitivity to RGCs. (A) Representative photocurrents recorded from a retinal ganglion cell in response to a 100 ms light pulse at different intensities (15.1 to 16.3 log photons cm2 s−1); a monoexponential function was fit to the tail currents at the end of the light pulse to calculate the off kinetics (τoff). (B) Distribution of τoff quantified in retinal ganglion cells (n = 16) (C) Photocurrent (left) and voltage (right) traces elicited by 1 ms pulses of different intensity (14.8 to 15.1 log photons cm2 s−1). Intensities above 14.98 photons cm2 s−1generated photocurrents that activated voltage-gated sodium channels, as evidenced by the large, transient inward currents. The amplitude of the voltage response increased with increasing intensities. In this cell, intensities equal to (and above) 15 log photons cm2 s−1 elicited action potentials. (D) Threshold intensity required to elicit an action potential for a 1 ms light pulse (n = 14 cells). All responses were elicited by full-field illumination with λ = 565 nm following bleaching by intense white light (see methods).
