Figure 6

bReaChES restores visually-evoked responses in dystrophic mice. (A) Looming stimulus enclosure set-up. Individual mice are placed in an arena with a central dish and shelter placed in one corner. An LCD panel is positioned overhead to present stimuli. Behaviour is monitored and recorded from below using a video camera (see Methods). Top inset: schematic showing expanding stimulus presented overhead. Panels show cross-sectional images of "looming" disk. Timeline: time course of a single trial. After a period of habituation, subjects are presented with an expanding positive contrast disk (white disk (15.7 log photons cm⁻² s⁻¹) expanding at rate of 72°s⁻¹ across 500 ms, with a subsequent maintenance of 500 ms (see Methods)). (B) Heat maps of individual mice showing instantaneous velocities 2 s before and after stimulus initiation. Note the arrest in movement (dark regions) exhibited by three of the four groups tested (WT-eYFP (WT-eY), WT-bReaChES (WT-bR), and Dys-bReaChES (Dys-bR)). (C) Mean velocities of movement 2 s before (pre) and 2 s after (post) stimulus onset. Dys-eYFP (Dys-eY) mice exhibited no detectable differences in motion. In contrast, Dys-bR subjects showed recovery of visually-evoked arrest. (N = 9 bReaChES-injected dys mice, N = 8 control-injected dys mice, N = 5 bReaChES-injected WT mice and N = 8 control-injected WT mice). Error bars indicate ± SD. *p < 0.05; **p < 0.01; ***p < 0.001, one-way ANOVA with Bonferroni post hoc test.