Figure 4
From: Anti-biofilm activity of caffeine against uropathogenic E. coli is mediated by curli biogenesis

Effects of caffeine on CFT073 andK12 MG1655 biofilms as determined by fluorescence microscopy (FM). Syto9 stained live bacteria and emit green fluorescence intensity. FM images ofCFT073 biofilms, (a) without caffeine; (b) with 24 mM caffeine; (c) with 48 mM caffeine; (d) quantitative analysis of fluorescence image ofCFT073 biofilm using ImageJ and GraphPad prism8. FM images of K12 MG1655 biofilms (e) without caffeine; (f) with 24 mM caffeine; (g) with 48 mM caffeine; (h) quantitative analysis of fluorescence image of K12 MG1655 biofilm using ImageJ and GraphPad prism8.