Figure 6

The expression of DLGAP4 in clinical specimens and cell lines of HCC. (A) Representative images of DLGAP4 staining in HCC tissues and adjacent normal tissues shown by haematoxylin–eosin staining (HE) and immunohistochemistry (IHC). (B) Western blot indicating the expression of DLGAP4 in HCC tissues and matching adjacent normal tissues. β-actin was used as an internal control. (C, D) mRNA and protein levels of DLGAP4 in a normal live cell line and two human HCC cell lines detected by RT–qPCR and Western blot, respectively. The data were obtained from the average of three independent experiments. *P < 0.05.