Figure 7

DLGAP4 knockdown suppresses the proliferation and growth of HCC cells in vitro. (A) Western blot analyses showing the expression levels of DLGAP4 in HepG2 and HCCLM3 cells stably transfected with shDLGAP4 or shNC. β-actin was used as a loading control. (B) CCK-8 assays revealed that shDLGAP4 inhibited the proliferation of HepG2 and HCCLM3 cells. (C) Representative micrographs of BrdU incorporation in shDLGAP4 and control cells. (D) Transwell migration assays of HepG2 cells and HCCLM3 cells transfected with shNC or shDLGAP4. The data were obtained from the average of three independent experiments. *P < 0.05.