Figure 3
From: A new negative feedback mechanism for MAPK pathway inactivation through Srk1 MAPKAP kinase
Atf1 response to stress is independent of Srk1. (a) Total extracts from growing cultures of wild-type and srk1∆ cells expressing genomic atf1:HA6his were untreated (0 min) or treated with 0.8 M KCl at the indicated times and resolved in SDS-PAGE. Total Atf1 protein was detected with anti-HA antibody. (b) atf1 deleted cells with (+) or without (−) Srk1 overexpression (OP Srk1: through the inducible plasmid pREP1-srk1:HA6his) for 20 h were untreated or treated with 0.8 M KCl for 15 min. Activated (Sty1-P) and total Sty1 were detected with anti-phospho p38 and anti-HOG1 antibodies, respectively. The level of overexpressed Srk1 was detected with anti-HA antibody. Anti-Cdc2 was used as a loading control (a and b). All blot membranes were cut prior antibodies incubation.
